rabbit polyclonal antiinos antibody  (Proteintech)

Journal: Journal of the American Heart Association

Article Title: Vasculogenic Conditioning of Peripheral Blood Mononuclear Cells Promotes Endothelial Progenitor Cell Expansion and Phenotype Transition of Anti‐Inflammatory Macrophage and T Lymphocyte to Cells With Regenerative Potential

doi: 10.1161/jaha.113.000743

Figure Lengend Snippet: Figure 10. Histological evaluation of inflammation in ischemic hindlimbs. A and C, Representative pictures of inflammation in ATM by immunohistochemistry using anti-iNOS antibody (brown color) in each group. 920 HPF. (a) Control of rabbit polyclonal IgG, (b) IMDM control, (c) PBMNCTx, and (d) QQMNCTx in (A). (a) Control of rabbit polyclonal IgG, (b) IMDM control, (c) eEPCTx, (d) QQMNCTx, and (e) GmCD34Tx in (C). B and D, The graphs show percent (%) iNOS expressing area in each group. *P<0.05; ***P<0.001 versus IMDM control in (B and D). #P<0.05 versus PBMNCTx in (B) or eEPCTx in (D). Each graph column represents a meanSE. N=6 mice per group. ATM indicates anterior tibial muscle; eEPCTx, early endothelial progenitor cell transplantation; GmCD34, granulocyte colony-stimulating factor mobilized CD34+ cell; HPF, high power field; iNOS, inducible nitric oxide synthase; PBMNCTx, peripheral blood mononuclear cell transplantation; QQMNCTx, quality and quantity control culture of mononuclear cell transplantation.

Article Snippet: Paraffin tissue sections were deparaffinized, autoclaved in citrate buffer at 121°C for 10 minutes to retrieve the target antigen, incubated with the primary Ab of rabbit polyclonal antiiNOS Ab (Abcam, Cambridge, MA) prediluted with 1% BSA/PBS (1:100 ratio) at 4°C overnight (Table 8).

Techniques: Immunohistochemistry, Control, Expressing, Transplantation Assay